Retinal Pigment Epithelium (RPE) Segmentation and Intensity
The retinal pigment epithelium (RPE) is a specialized cell monolayer that forms the barrier between the subretinal and the choroidal spaces. We used high-content imaging, machine-learning-based image-segmentation, phenotypic quantification, and mathematical modeling to develop quantitative 3-dimensional maps of subcellular structures during the establishment of RPE apical/basal polarity.
This collection contains:
1) volumetric images of induced pluripotent stem cell-derived RPE (iPSC-RPE) differentiated from sixteen isogenic iPSC lines where specific proteins were tagged with a fluorophore.
2) volumetric images of cell and nuclear border segmentation and tagged-protein segmentation.
3) a software to run the entire analysis starting from the fluorecence image stacks.
4) a software to morph cells and organelles to calculate spatial interaction maps.
CITE THIS COLLECTION
FUNDING
Developing functional and authenticated RPE tissue from iPS cells
National Eye Institute
Research Institution(s)
National Eye Institute; National Institute of Standards and Technology; National Center for Advancing Translational Sciences; National Cancer InstituteContact email
kapil.bharti@nih.govAssociated Preprint DOI
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AUTHORS (16)
- PSPushkar SatheAVAndrei VolkovDRDominik ReichertSSSheldon SebastianAMArvydas MaminishkisNSNicholas ShaubBLBengt LjungquistDBDevika BoseJFJorge FerrariNLNyusha LinCSCarl SimonRSRuchi SharmaPBPeter BajcsyKBKapil Bharti
