FigS4-Qu+al.gif

Animated GIF showing further examples of phenotypes induced by Shot-PE-Life::GFP expression. Primary neurons at 6-8 HIV on glass with scabrous-Gal4-induced expression of Shot-PE-Life::GFP, stained for tubulin (green), actin (red) and GFP (blue); the animation sequence shows single channels as grayscale images, as indicated top left in animation steps. Symbols indicate the following: asterisks, cell bodies; arrowheads, MT bundle split; arrows, 'tennis racket' spools; white curved arrows, unusual MT bundle malformations; open curved arrows, unusually bundled MTs in cell bodies.