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Qualitative Multiresidue Screening Method for 143 Veterinary Drugs and Pharmaceuticals in Milk and Fish Tissue Using Liquid Chromatography Quadrupole-Time-of-Flight Mass Spectrometry
journal contribution
posted on 2015-05-13, 00:00 authored by Marilena
E. Dasenaki, Anna A. Bletsou, George A. Koulis, Nikolaos S. ThomaidisA wide-scope
screening methodology has been developed for the identification
of veterinary drugs and pharmaceuticals in fish tissue and milk using
ultrahigh-performance liquid chromatography quadrupole time-of-flight
mass spectrometry (UHPLC-QTOF MS). The method was validated using
a qualitative approach at two concentration levels. The detection
of the residues was accomplished by retention time, accurate mass,
and the isotopic fit using an in-house database. Product-ion spectra
were used for unequivocal identification of the compounds. Generic
sample treatment was applied. The majority of the compounds were successfully
detected and identified at concentration levels of 150 ng mL–1 in milk and 200 μg kg–1 in fish (>80%
of
the compounds in both matrices), whereas satisfactory results were
also obtained at concentration levels of 15 ng mL–1 in milk and 20 μg kg–1 in fish (>60%
of
the compounds detected and identified).