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Molecular Effects and Tissue Penetration Depth of Physical Plasma in Human Mucosa Analyzed by Contact- and Marker-Independent Raman Microspectroscopy
journal contribution
posted on 2019-11-08, 22:29 authored by Thomas Wenzel, Daniel A. Carvajal Berrio, Ruben Daum, Christl Reisenauer, Klaus-Dieter Weltmann, Diethelm Wallwiener, Sara Y. Brucker, Katja Schenke-Layland, Eva-Maria Brauchle, Martin WeissNoninvasive
epithelial tissue treatment with cold atmospheric plasma
(CAP) is a promising option for local treatment of chronic inflammatory
and precancerous lesions as well as various mucosal cancer diseases.
Atmospheric pressure plasma jets (APPJ) are well-characterized and
medically approved plasma sources. There are numbers of medically
approved plasma sources for the treatment of epithelial diseases;
however, little is known about the biochemical effects of CAP at the
plasma–tissue interface. Furthermore, the actual penetration
depth of CAP into tissue is currently unclear. Noninvasive and marker-independent
Raman microspectroscopy was employed to assess the molecular effects
of CAP on single cells and primary human cervical tissue samples.
CAP treatment showed immediate and persisting changes of specific
molecular tissue components determined by multivariate analysis. Raman
imaging identified CAP-dependent changes in the morphology of the
tissue, as well as molecular tissue components. The expression of
the different components was not significantly altered within 24 h
of incubation. DNA and lipids showed the strongest changes upon CAP
treatment, which were traced to the basal cell layer of cervical epithelium,
corresponding to an average functional plasma penetration depth of
roughly 270 μm. In this study, Raman microspectroscopy is shown
to be a promising method for molecular single-cell and solid tissue
characterization. Regarding CAP treatment of tissues, Raman microspectroscopy
could be suitable for the screening of biological mechanisms as well
as for future contact- and marker-independent monitoring of plasma
tissue effects.
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Keywords
marker-independent Raman microspectroscopybasal cell layerCAP treatmentplasma sourcesHuman Mucosa Analyzed270 μ mMarker-Independent Raman Microspectroscopy Noninvasive epithelial tissue treatmentAtmospheric pressure plasma jetsTissue Penetration DepthAPPJmucosal cancer diseasestissue componentsRaman microspectroscopyplasma tissue effectsDNAplasma penetration depth
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