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Inhibition of fungal pathogens across genotypes and temperatures by amphibian skin bacteria

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posted on 2017-08-10, 14:29 authored by Carly Muletz WolzCarly Muletz Wolz, Jose G. Almario, Samuel E. Barnett, Graziella DirenzoGraziella Direnzo, An Martel, Frank Pasmans, Kelly R. Zamudio, Luis F. Toledo, Karen LipsKaren Lips
Dataset consists of two sets of data files with associated R code:

1a) Raw plate read data titled "final_all_plates_gabe_no_contam.csv". This file consists of all OD readings for every well for every day that readings were taken. We used R code to correct baseline zoospore OD, fit a linear regression to the OD readings over time, remove wells with low r2 values, and calculate inhibition scores

1b) R code to work with the "final_all_plates...csv is titled "Calculate_score_inhibit_final.R"

2a) The inhibition scores for each well titled "Inhibition_scores.final.csv". This is the output of the R code above. We did edit this file in excel to add a genotype column that is not in the R code

2b) The averages of inhibition scores used to make the heatmap figure titled "heatmap_averages_final.csv". See more details in the associated .Rmd file to see how we generated these values.

2c) R code to do analyses of inhibition scores and make figures titled "Publish_Bac_Temp_BdBsal.Rmd"

2d) The knitted .Rmd file titled "Publish_Bac_Temp_BdBsal.html"

Funding

NSF REU supplement to grant DEB #1120161 to KRL and KRZ

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