Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Video Clip S1+S2

Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Material.

Frederik Holst & Christian Ruiz

The supplementary material refers to:

Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (ESR1) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007).

Subsequent Supplementary Figures S1-S7

Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented ESR1 copy number profiles of 22 breast cancers. A photo documentation of ESR1 FISH analysis signal appearance (figures 5A-C + 7A-C) of tumors with ESR1 amplification by SNP GeneChip analysis is added. 

DOI: http://dx.doi.org/10.6084/m9.figshare.1470035

Subsequent Supplementary Video Clips S1+S2

The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. 

DOI: http://dx.doi.org/10.6084/m9.figshare.1471677

Subsequent Supplementary Optical Dataset S1

The optical dataset consists of 94 photographs documenting appearance of ESR1 FISH analysis signals of 20 out of 22 tumors regarding SNP GeneChip analysis illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. 

DOI: http://dx.doi.org/10.6084/m9.figshare.1470130

Methods References

Ruiz, C. Doctoral Thesis, Universität Basel (2006).

Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. & van Diest, P.J. ESR1 Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013).

Holst, F. FISH Analysis of ESR1 Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).