Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Video Clip S1+S2

2015-08-23T09:07:29Z (GMT) by Frederik Holst Christian Ruiz
<p><strong>Estrogen receptor alpha (<em>ESR1</em>) gene amplification is frequent in breast cancer. Subsequent Supplementary Material.</strong></p> <p>Frederik Holst & Christian Ruiz</p> <p>The supplementary material refers to:</p> <p>Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (<em>ESR1</em>) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007).</p> <p>Subsequent Supplementary Figures S1-S7</p> <p>Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented <em>ESR1</em> copy number profiles of 22 breast cancers. A photo documentation of ESR1 FISH analysis signal appearance (figures 5A-C + 7A-C) of tumors with <em>ESR1</em> amplification by SNP GeneChip analysis is added. </p><p>DOI: http://dx.doi.org/10.6084/m9.figshare.1470035</p> <p>Subsequent Supplementary Video Clips S1+S2</p> <p>The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. </p><p>DOI: http://dx.doi.org/10.6084/m9.figshare.1471677</p> <p>Subsequent Supplementary Optical Dataset S1</p> <p>The optical dataset consists of 94 photographs documenting appearance of <em>ESR1</em> FISH analysis signals of 20 out of 22 tumors regarding SNP GeneChip analysis illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. </p><p>DOI: http://dx.doi.org/10.6084/m9.figshare.1470130</p> <p><strong>Methods References</strong></p> <p>Ruiz, C. Doctoral Thesis, Universität Basel (2006).</p> <p>Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. & van Diest, P.J. <em>ESR1</em> Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013).</p> <p>Holst, F. FISH Analysis of <em>ESR1</em> Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).</p>