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Viability observations of E.coli O157:H7 by differential /double staining of living and dead cells after SAEW treatment under a confocal microscope (A). The live-dead percentage of E.coli under SAEW treatment (60 ppm) was assessed with a flow cytometry at the following: (B) Control (Unstained), (C) Live cells (D) Dead cells. Live and dead cells percentage (%) under SAEW treatment were represented as a chart (E). SAEW treatment resulted in retaining E,coli morphological changes. (F) a Live

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modified on 2018-04-11, 21:31

SYT-PI Single and double staining of live and dead bacterial cells of E.coli O157:H7 and S. aureus were used to prove the impact of SAEW on bacterial viability condition under a confocal laser-scanning microscopy (Fig. 3A and 4A). Live cells were represented as green fluorescence (Fig. 3F) and dead cells were represented as red fluorescence (Fig. 3G and 3H).