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CRISPR mediated mutagenesis results of rol-6 homologues results in a distinct right roller phenotype in A. rhodensis and S. stercoralis.
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modified on 2018-09-25, 15:59 Videos illustrating that CRISPR-liposome mediated mutagenesis of Arh-rol-6.1 (a homologue of the cuticle collagen gene rol-6 in C. elegans) results in a distinct right roller phenotype.
The phenotype is apparent in the P0 nematode
(CRISPR-Arh-rol-6_1_P0) and is faithfully inherited by all the F1 and F2 progeny (CRISPR-Arh-rol-6_1_F2_from_single_F1_line1 and CRISPR-Arh-rol-6_1_F2_from_single_F1_line2). This roller movement contrasts with the the sinusoidal pattern of movement of wild-type A. rhodensis nematodes (WT_mixed_population).
