Dataset: High-throughput screening identifies selective synergistic drug combinations in 2D and 3D colorectal cancer cell cultures

High-throughput screening dataset generated to compare drug combination effects in 2D and 3D culture models of three CRC cell lines (HCT-116,
HT-29 and SW-620). The combinatorial treatments investigated comprised all pairwise combinations of five experimental targeted small molecule inhibitors (olaparib, PD0325901, palbociclib, PI-103, 5Z-7-oxozeaenol) and two approved chemotherapeutic drugs (5-FU, oxaliplatin). The dataset contains data from three consecutive screens:

1. Single-drug dose response screen (SD), where cells were treated with the drugs in single application (48h).

2D response was evaluated by assessing:
- viability (CellTiter-Glo 2.0) - Supplementary file 1
- confluency (imaging) - Supplementary file 2
- apoptosis (NucView 488 Caspase 3 substrate) - Supplementary file 3

- cell death (CellTox Green) - Supplementary file 4

3D response was evaluated by assessing:
- viability (CellTiter-Glo 3D) - Supplementary file 5
- size (imaging) - Supplementary file 6

2. Combination screen (CS) where cells were treated with all the drugs in single- and all pairwise combinations in 4x4 dose matrices (48h).

2D response was evaluated by assessing:
- viability (CellTiter-Glo 2.0) - Supplementary file 7-9, Additional file 1
- confluency (imaging) - Supplementary file 10-12, Additional file 5

3D response was evaluated by assessing:
- viability (CellTiter-Glo 3D) - Supplementary file 13-15, Additional file 2
- size (imaging) - Supplementary file 16-18, Additional file 6

Combination effects were evaluated based on the Bliss independence model for each readout separately:

- viability 2D - Supplementary file 19, Additional file 3
- confluency 2D - Supplementary file 20, Additional file 7

- viability 3D - Supplementary file 21, Additional file 4

- size 3D - Supplementary file 22, Additional file 8

3. Long-term screen (LT) where cells were treated with a subset of the drugs tested in CS for 96h.

2D response was evaluated by assessing:
- viability (CellTiter-Glo 2.0) - Supplementary file 23-26, Additional file 11
- confluency (imaging) - Supplementary file 27-30, Additional file 15
- apoptosis (NucView 488 Caspase 3 substrate) - Supplementary file 31, Additional file 10

3D response was evaluated by assessing:
- viability (CellTiter-Glo 3D) - Supplementary file 32-35, Additional file 12
- size (imaging) - Supplementary file 36-39, Additional file 16
- apoptosis (NucView 488 Caspase 3 substrate) - Supplementary file 40-43, Additional file 9

Combination effects were evaluated based on the Bliss independence model for viability 2D and 3D:

- viability 2D - Supplementary file 44, Additional file 13

- viability 3D - Supplementary file 45, Additional file 14