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ACA DGE data(Single cell RNA-seq part_larva neotenic axolotls)

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modified on 2022-08-19, 06:49

Mexican axolotl (Ambystoma mexicanum) is a promising tetrapod model in regeneration and developmental studies. Neotenic axolotl rarely undergoes spontaneous metamorphosis. A system single-cell analysis of neotenic and metamorphosed axolotl molecular characteristics is still lacking. During thyroxine-induced metamorphosis in adult axolotls, regeneration ability and lifespan may gradually decline. These phenotypes may cause by cell type transition and gene expression perturbation. Here, we adopted a combinatorial hybridization based single-cell RNA-seq and single-nucleus ATAC-seq method to generate tissue based multi-omics atlas of adult axolotl. Gene expression and chromatin accessibility profiling of ~1.2 million single cells and nuclei across 19 tissues constructed the first adult axolotl cell atlas. Single-cell chromatin accessibility dataset defined critical regulators and its motif in neotenic axolotl tissues. Comparison between neoteny and metamorphosis tissues revealed transcriptome heterogeneity of structural cells in different tissues and their sophisticated regulatory network. Furthermore, we described dynamic gene expression pattern during neotenic axolotl limb development. These data serve as a rich resource to explore molecular identity of axolotl as well as its metamorphosis.

Funding

This work was supported by the National Natural Science Foundation of China (grants 31930028, 31922049, 91842301), the National Key Research and Development Program (grants 2018YFA0800503, 2018YFA0107804, 2018YFA0107801), and the Fundamental Research Funds for the Central Universities (G.G.).