Antimicrobial activity of slightly acidic electrolyzed water (SAEW) treatment against bacterial pathogens.

Antimicrobial properties of slightly acidic electrolyzed water (SAEW) treatment was evaluated against Gram positive and Gram negative bacterial pathogens and the results were shown in Figure 6. The results revealed that different concentrations (20, 40, and 60 ppm) of SAEW showed the antimicrobial activity. Among the SAEWs, SAEW containing 60 ppm was the most effective retarding microbial growth of pathogens. Distilled water (without treatment) did not show the antimicrobial activity. SAEW containing 20, 40, and 60 ppm represented 0.9 to 1.2 mm zone of inhibition against S. aureus (ATCC 13150) and 1.2 to 1.6 mm zone of inhibition against Salmonella (ATCC 14028). In addition, SAEW retaining 20, 40, and 60 ppm indicated 1.3 to 1.5 mm of inhibition zone for E. coli O157:H7 (ATCC 35150) and 1.3 to 1.8 mm for B. cereus (ATCC 14579).