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ACA DGE data (Single-cell RNA-seq part for adult animals)
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modified on 2022-08-19, 08:13 Mexican
axolotl (Ambystoma mexicanum) is a promising tetrapod model in regeneration and
developmental studies. Neotenic axolotl rarely undergoes spontaneous
metamorphosis. A system single-cell analysis of neotenic and metamorphosed
axolotl molecular characteristics is still lacking. During thyroxine-induced
metamorphosis in adult axolotls, regeneration ability and lifespan may gradually
decline. These phenotypes may cause by cell type transition and gene expression
perturbation. Here, we adopted a combinatorial hybridization based single-cell
RNA-seq and single-nucleus ATAC-seq method to generate tissue based multi-omics
atlas of adult axolotl. Gene expression and chromatin accessibility profiling
of ~1.2 million single cells and nuclei across 19 tissues constructed the first
adult axolotl cell atlas. Single-cell chromatin accessibility dataset defined
critical regulators and its motif in neotenic axolotl tissues. Comparison
between neoteny and metamorphosis tissues revealed transcriptome heterogeneity of
structural cells in different tissues and their sophisticated regulatory network.
Furthermore, we described dynamic gene expression pattern during neotenic
axolotl limb development. These data serve as a rich resource to explore molecular
identity of axolotl as well as its metamorphosis.