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TCR beta repertoires in naive CD4+ T cell subsets in identical twin donor samples

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dataset
modified on 30.01.2020, 11:31

TCR repertoire datasets for sorted naive CD4+ T cell subsets.


Includes metadata and preprocessed clonesets for sorted naive CD4+ T cell subsets: naive Treg, CD31+-enriched (recent thymic emigrates, RTE subset), CD31- naive subset (non-RTE subset, mature naive CD4+ T cells).

Naive T cell subsets were sorted from 5 pairs of identical twins, all 28-35 years old males; and two more healthy donors (not twins).

Each sample is presented in txt file: TCR beta cloneset tab-delimited table, VDJtools software format.


Raw sequencing fastq data was processed with using MIGEC v.1.2.9 (threshold 1 UMI for CDR3-containing clonotype), aligned with MiXCR v.2.1.1. In-frame TCR CDR3 repertoires were extracted with VDJtools v. 1.2.1.

Funding

Ministry of Science and Higher Education of the Russian Federation grant (075-15-2019-1789)