gene.expression.symbol.txt

Total RNA from U251MG, U87MG, U251TR and U87TR cell lines were extracted and quanlified. A total amount of 3 μg RNA per sample was used as input material for library preparation. The library preparations went through standard Illumina Hiseq platform and 150 bp paired-end reads from transcriptome sequencing were generated. After quality control procedures, the data were mapped to human hg38 reference by STAR (Dobin et al., 2013). And then the expression level (FPKM) of each gene was calculated based on the length of the gene and reads count mapped to this gene.