Figure S3. Palladin Recruitment at L. monocytogenes Requires ActA.

HeLa cells were infected with L. monocytogenes strain ΔactA for 6 hours, fixed and stained with a mouse monoclonal palladin targeting antibody (green), DAPI (blue) to visualize DNA and Alexa594-phalloidin (red) to visualize actin. Palladin is absent from the surface of intracellular bacteria (boxed regions). Insets (enlargement of boxed regions) points to an individual bacterium (open arrowhead). Scale bar, 10 µm and 2 µm (insets).