Trinity_longorf_pep.fasta.zip

The 23,372 ESTs of E. gracilis available at the NCBI (as of May 24, 2012) were contiged with CAP3 and then translated in the reading frame yielding the longest protein fragment (using BioPerl longorf.pl script), whereas ~250-Mbp of 454 reads was assembled with the Velvet de novo assembler [version 1.2.03] and then translated in the same way as EST contigs.