Glycogen levels in midgut gland of Lithodes santolla after starvation
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modified on 2017-08-30, 13:45 Male king crab (Lithodes santolla) were collected from the Beagle Channel (Tierra del Fuego, southern Argentina) using baited traps and fishing boats.Twenty four king crabs in
intermoult stage (114 mm LC) were
placed in individual containers with chilled seawater (6±1°C) under continuous
aeration. The first day of the assay (time 0, initial control), 6 animals were dissected and their midgut gland was extracted. The remaining crabs were not fed until day 30, 45 or 60. At each time, 6 animals were randomly dissected.
Glycogen
concentration in the midgut gland was measured based on Lo´s
protocol (Lo et al 1970).