Data undelying the research on: New culture medium for micropropagation protocol of guava (Psidium guajava) seedlings, a plant with interest in cool subtropics.docx

Psidium guajava is a new crop cultivated in Greece, with a potential pharmaceutical and ornamental uses. An efficient and reliable protocol for in vitro clonal propagation of P. guajava from seeds was developed. Five different media were examined. Shoot proliferation succeeded on a new basal medium (BB) with extra vitamin solution, without plant growth regulators. In addition, shoots on media supplemented with BB vitamins solution performed better than in MS and WP media. When BB medium was used, the greatest shoot number resulted in 4 mg L-1 BA with a score of 2.8. Regenerated single shoots were rooted in the BB medium, supplemented with 0.1 ΝΑΑ (mg L-1) and producing 2.2 roots of 4.6 cm, along with 3.1 cm of adventitious shoots. Acclimatization was accomplished in three phases, using peat-moss, vermiculite and perlite (1:1:1), declining a total survival of 83.3%. In order to examine the genetic diversity of the plant material, ISSR and SCoT molecular markers were used. The results indicated a low diversity, along with high percentage of polymorphism for both ISSR and SCoT analyzes.