Supplemental data.pdf

Total RNA was isolated from the hearts of male Sprague Dawley rats treated with normal drinking water or 3% DSS. miRNA profiling was done using microRNA microarrays (Microarray Version 21, MRA-1003; LC Sciences, Houston, TX). Data were analyzed by subtracting the background and then normalizing the signals using a LOWESS (Locally-Weighted Regression) filter. The miRNA transcript was considered as reliably detectable only if the signal intensity was greater than 3 times the background standard deviation; the spot coefficient of variation was < 0.5, and at least 50% of the repeated probe signals were above the detection level.