Targeted PCR on vitreous DNA - (Item H)

In another independent assessment regarding the most abundant organism in the vitreous samples we employed targeted PCR assays to detect the two most frequently identified bacteria, Staphylococcus epidermidis and Enterococcus faecalis. For the detection of S. epidermidis the species-specific primers SE705-1 (5’-ATC AAA AAG TTG GCG AAC CTT TTC A-3’) and SE705-2 (5’-CAA AAG AGC GTG GAG AAA AGT ATC A-3’) were used (Martineau et al. 1996). For the detection of E. faecalis the species-specific primers ddl-E1 (5’-ATC AAG TAC AGT TAG TCT-3’) and ddl-E2 (5’-ACG ATT CAA AGC TAA CTG-3’) were used (Dutka-Malen et al., 1995).

Reference

Kirstahler P, Bjerrum SS, Friis-Møller A, la Cour M, Aarestrup FM, Westh H., and Pamp SJ. (2018) Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid. Scientific Reports, doi:10.1038/s41598-018-22416-4.