The functional dark side

Please visit https://dark.metagenomics.eu for a detailed description of the project. In brief, we developed a conceptual model and a computational workflow to unify the known and the unknown coding sequence space and thus, facilitate the integration of the unknown genes in microbial analyses. We implemented our approach on a comprehensive dataset of metagenomic and genomic data, from which built a dataset of gene clusters (GCs) functionally partitioned in categories, and environmentally and phylogenetically contextualised. The seed dataset (agnostosDB_dbf02445-20200519) was build using 1,749 metagenomes (from marine and human microbiome) and 28,941 bacterial and archaeal genomes. The computational workflow we developed allows the integration of new genomic and metagenomic data. The workflow is publicly available in a GitHub repository at https://github.com/functional-dark-side/agnostos-wf.