Supplemental Material for "Evaluating mtDNA metabarcoding markers to identify zooplankton and ichthyoplankton in the Salish Sea: Resolution in comparison to morphology"

<p>Supplemental material for the manuscript: "Evaluating mtDNA metabarcoding markers to identify zooplankton and ichthyoplankton in the Salish Sea: Resolution in comparison to morphology".</p> <p><br></p> <p>Includes supplemental materials mentioned in the manuscript:</p> <ul> <li>Supplemental Figure 1: Krona plots</li> <li>Supplemental Figure 2: Species trees showing detection resolution by marker/morphology for A) Decapoda, B) Cnidaria, C) Echinodermata, D) Gastropoda, and E) Teleost bony fish.</li> <li>Supplemental Figure 3: Correlations between relative proportions of arthropod species from morphological analysis (biomass, dry weight) versus sequence reads per sample, from a general invertebrate metabarcoding marker, LrCOI.</li> <li>Supplemental Table 1: Metabarcoding markers and gene regions, references, primer sequences, approximate target gene region length, and PCR parameters.</li> <li>Supplemental Table 2: Read/read pair counts for each marker/sample combination through all steps in the quality control bioinformatics pipeline.</li> <li>Supplemental Table 3: Master dataset showing relative abundance per sample for each unique taxa, separated by marker.</li> </ul> <p>Supplemental data for the record:</p> <ul> <li>Marker gene data sets. ASV information per marker.</li> <li>Morphology data sets. Density and biomass estimates based on taxonomic identification using plankton morphology.</li> <li>Marker comparison to Morphology Biomass relative abundance estimates.</li> </ul>