Supplemental Material for "Evaluating mtDNA metabarcoding markers to identify zooplankton and ichthyoplankton in the Salish Sea: Resolution in comparison to morphology"

Supplemental material for the manuscript: "Evaluating mtDNA metabarcoding markers to identify zooplankton and ichthyoplankton in the Salish Sea: Resolution in comparison to morphology".

Includes supplemental materials mentioned in the manuscript:

• Supplemental Figure 1: Krona plots
• Supplemental Figure 2: Species trees showing detection resolution by marker/morphology for A) Decapoda, B) Cnidaria, C) Echinodermata, D) Gastropoda, and E) Teleost bony fish.
• Supplemental Figure 3: Correlations between relative proportions of arthropod species from morphological analysis (biomass, dry weight) versus sequence reads per sample, from a general invertebrate metabarcoding marker, LrCOI.
• Supplemental Table 1: Metabarcoding markers and gene regions, references, primer sequences, approximate target gene region length, and PCR parameters.
• Supplemental Table 2: Read/read pair counts for each marker/sample combination through all steps in the quality control bioinformatics pipeline.
• Supplemental Table 3: Master dataset showing relative abundance per sample for each unique taxa, separated by marker.

Supplemental data for the record:

• Marker gene data sets. ASV information per marker.
• Morphology data sets. Density and biomass estimates based on taxonomic identification using plankton morphology.
• Marker comparison to Morphology Biomass relative abundance estimates.