This pipeline was created by Pearl V. Ryder and Dorothy A. Lerit to investigate the distribution of subcellular structures in fluorescence microscopy data in three dimensions.
This dataset provides raw single-channel tif images of centrosomes and RNA to test the pipeline. Sample results files are provided to compare to your results.
The SubcellularDistribution pipeline code and tutorial are available at: https://github.com/pearlryder/subcellular-distribution-pipeline