Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH
Data related to the EEL FISH method manuscript.
Read the paper here: https://www.nature.com/articles/s41587-022-01455-3#Sec31
Methods to spatially profile the transcriptome are dominated by a trade-off between resolution and throughput. Here, we develop a method named EEL FISH that can rapidly process large tissue samples without compromising spatial resolution. By electrophoretically transferring RNA from a tissue section onto a capture surface, EEL speeds up data acquisition by reducing the amount of imaging needed, while ensuring that RNA molecules move straight down towards the surface, preserving single-cell resolution. We apply EEL on eight entire sagittal sections of the mouse brain and measure d the expression patterns of up to 440 genes to reveal complex tissue organisation. Moreover, EEL can be used to study challenging human samples by removing autofluorescent lipofuscin, enabling the spatial transcriptome of the human visual cortex to be visualized. We provide full hardware specification, all protocols and complete software for instrument control, image processing, data analysis and visualization.