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Phallusia mammillata embryonic development

Published on by Patrick Lemaire

In this project, we describe the embryonic development of the ascidian Phallusia mammillata using multi-view Light-sheet (MuVi-SPIM) imaging of embryos whose cell membranes were fluorescently labelled. Each cell was then automatically segmented and tracked in time using the ASTEC pipeline.

The data shown are described in : 

  • Guignard, Fiuza et al., (2020) Contact area–dependent cell communication and the morphological invariance of ascidian embryogenesis Science, Vol. 369, Issue 6500, eaar5663 DOI: 10.1126/science.aar5663.
  • Biasuz et al, (2023) A framework for systematic automated cell naming in segmented ascidian embryos In prep.

For some embryos (ASTEC-Phmamm1/3/4/5/7/8/9) 2 different datasets are shared: one corresponding to the 2020 publication version and a second corresponding to the 2023 publication version (identified by the suffix "-v2") . For these embryos, segmentation images and/or properties (especially cell names) have been edited. For further information, please consult Biasuz et al., 2023. 

Please also note that the nomenclature "Astec-Pm" has been changed to "Astec-Phmamm" to match the nomenclature of ANISEED (www.aniseed.fr), the reference database for ascidians.  

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Funding

CNRS, Université de Montpellier, INRIA, Agence Nationale de la Recherche, Fondation Schueler Bettencourt, Fondation pour la Recherche Médicale

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