JB
High-throughput Transcriptomic and Proteomic Profiling of Mesenchymal-Amoeboid Transition in 3D Collagen
Published on by Jan Brábek
We performed transcriptomic and proteomic profiling of HT1080 fibrosarcoma cells undergoing mesenchymal-amoeboid transition (MAT) in 3D collagen-based cultures. Two approaches were used to induce MAT: doxycycline-inducible constitutively active RhoA expression and dasatinib treatment. RNA sequencing was performed with ribo-depleted total RNA. Protein samples were analysed with tandem mass tag (TMT)-based mass spectrometry.
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Funding
The Kellner Family Foundation Principal Investigator Grant
Center for Tumor Ecology (reg. no. CZ.02.1.01/0.0/0.0/16_019/0000785) supported by the Operational Programme Research, Development and Education.
Charles University, projects GA UK No 712217 and 1292217
Czech Science Foundation grant 19-08410S
Equipment for metabolomics and cell analyses (Grant no. CZ.1.05/2.1.00/19.0400) supported by the Research and Development for Innovations Operational Program, co-financed by the European regional development fund and the state budget of the Czech Republic.
