3D-GROWTH-MDA-MB-231-MATRIGEL

In this series of experiments we examined the growth of MDA-MB-231 cell line (Triple Negative Breast Cancer) in 3D Matrigel scaffolds, in absence and presence of Paclitaxel.

Control datasets: 

-MDA-MB-231-MATRIGEL-SERIES-1

-MDA-MB-231-MATRIGEL-SERIES-2

Paclitaxel treatment datasets:

-MDA-MB-231-MATRIGEL-PACLITAXEL

Examined Paclitaxel doses: 0.5, 0.05, 0.005, 0.0005 μM

Examined time-points (days): 0, 2, 5, 7, 9, 12, 14

Paclitaxel administration time-point: Day 5

Total number of datasets:

-Control: 12 per time-point (6 in series 1, 6 series 2)

-Treatment: 20 per time-point (5 per dose)

Imaging modality: 

-Confocal microscope (Nikon A1R HD25)

Fluorescent label: GFP

Matrigel scaffold shape: semi-ellipsoid

Matrigel scaffold dimensions: 2.5 x 2.5 x 0.9 mm

Cell distribution in Matrigel: sparse and uniform

References:

Control datasets appear in:

• Dimitriou, N. M., Flores-Torres, S., Kinsella, J. M., & Mitsis, G. D. (2022). Quantifying the Morphology and Mechanisms of Cancer Progression in 3D in-vitro environments: Integrating Experiments and Multiscale Models. IEEE Transactions on Biomedical Engineering, 1–12. https://doi.org/10.1109/TBME.2022.3216231
• Dimitriou, N. M., Flores-Torres, S., Kinsella, J. M., & Mitsis, G. D. (2022). Detection and Spatiotemporal Analysis of In-vitro 3D Migratory Triple-Negative Breast Cancer Cells. Annals of Biomedical Engineering. https://doi.org/10.1007/s10439-022-03022-y

Control and Treatment datasets appear in:

• Dimitriou, N. M., Flores-Torres, S., Kyriakidou, M., Kinsella, J. M., & Mitsis, G. D. (2023). Cancer cell sedimentation in 3D cultures reveals active migration regulated by self-generated gradients and adhesion sites. BioRxiv. https://doi.org/10.1101/2023.02.15.528731