Using Enzymes
to Harvest Fatty Acids from Galactosyldiacylglycerols,
the Most Abundant Lipids in Plant Biomass
Posted on 2024-02-29 - 07:05
Mono- and digalactosyldiacylglycerols (MGDG, DGDG), the
main lipids
of plant photosynthetic membranes, represent a large but unexploited
reservoir of fatty acids on earth. They are dispersed in plant biomass
(milligrams per gram of dry mass) and not as accessible as vegetable
oils by simple physical means. The identification and characterization
of galactolipid acyl hydrolases, or galactolipases, raise the possibility
to use these biocatalysts for the bioconversion of galactolipids.
Here, we show that two enzymes of mammalian and microbial origins,
pancreatic lipase-related protein 2 from guinea pig (GPLRP2) and cutinase
from Fusarium solani, have the capacity to directly
and fully release the fatty acids of MGDG and DGDG present in various
plant leaves and green wastes. This high substrate accessibility to
enzymes was further explored by performing alcoholysis reactions in situ and showing the conversion of galactolipid fatty
acids into fatty acid alkyl esters (FAAE) when the enzyme and leaves
were incubated in the presence of 6 or 2.5 M ethanol. These findings
pave the way to the recovery of fatty acids dispersed in green biomass
and the exploitation of an additional and renewable source of fatty
acids for oleochemistry and nutrition in a context of competition
for vegetable oils.
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Amara, Sawsan; Lecomte, Jérôme; Barouh, Nathalie; Sahaka, Moulay; Lafont, Dominique; Rodier, Jean-David; et al. (2024). Using Enzymes
to Harvest Fatty Acids from Galactosyldiacylglycerols,
the Most Abundant Lipids in Plant Biomass. ACS Publications. Collection. https://doi.org/10.1021/acssuschemeng.3c07439
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AUTHORS (11)
SA
Sawsan Amara
JL
Jérôme Lecomte
NB
Nathalie Barouh
MS
Moulay Sahaka
DL
Dominique Lafont
JR
Jean-David Rodier
GP
Goetz Parsiegla
FD
Frédéric Demarne
BG
Brigitte Gontero
PV
Pierre Villeneuve
FC
Frédéric Carrière
KEYWORDS
simple physical meansperforming alcoholysis reactionsmilligrams per gramhigh substrate accessibilitygalactolipid acyl hydrolasesplant photosynthetic membranesharvest fatty acidsgalactolipid fatty acidsfusarium solani plant biomass monorelated protein 2various plant leavesfatty acids dispersedfatty acidsplant biomasssitu green biomassvegetable oilsunexploited reservoirrenewable sourcepancreatic lipasemicrobial originsmain lipidsguinea piggreen wastesfully releasefindings pavedry massdgdg presentdgdg ),abundant lipids