Using ANOVA Models To Compare and Optimize Extraction
Protocols of P3HBHV from Cupriavidus necator
Version 2 2016-09-29, 16:47
Version 1 2016-09-26, 13:51
Posted on 2016-09-14 - 00:00
Polyhydroxyalkanoates
(PHAs), produced by microorganisms, have
attracted considerable attention due to their biodegradability. However,
the cost for the production of PHA is still too high to be competitive
against petro-based polymers, greatly caused by the expensive downstream
processing (DSP). The DSP, moreover, is often ecologically unfriendly
due to usage of large amounts of highly volatile organic solvents.
To overcome these limitations, we systematically compared for the
first time seven different extraction protocols reported previously
using the same starting biomass of Cupriavidus necator. Design of experiments (DoE) and analysis of variance (ANOVA) models
were applied to further improve two of the most promising protocols.
Finally, we developed a method where a combination of mechanical disruption
of cells via bead milling with an addition of sodium lauryl sulfate
(SDS) was used. This method was optimized with a response surface
methodology and allowed a fast PHA extraction within approximately
2 h with a PHA recovery of almost 100% and PHA purity of 94%.
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Gutt, Beatrice; Kehl, Karl; Ren, Qun; Boesel, Luciano F. (2016). Using ANOVA Models To Compare and Optimize Extraction
Protocols of P3HBHV from Cupriavidus necator. ACS Publications. Collection. https://doi.org/10.1021/acs.iecr.6b02694
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AUTHORS (4)
BG
Beatrice Gutt
KK
Karl Kehl
QR
Qun Ren
LB
Luciano F. Boesel