Targeted Imaging of CD206 Expressing Tumor-Associated
M2-like Macrophages Using Mannose-Conjugated Antibiofouling Magnetic
Iron Oxide Nanoparticles
Posted on 2020-06-25 - 21:04
Although
tumor-associated macrophages (TAMs) have been shown to
promote cancer progression, their roles in tumor development and resistance
to therapy remain to be fully understood, mainly because of the lack
of a good approach to evaluate the dynamic changes of heterogeneous
macrophages in their residing microenvironment. Here, we report an
approach of using antibiofouling PEG-b-AGE polymer-coated
iron oxide nanoparticles (IONPs) for targeted imaging of mannose receptor
(CD206)-expressing M2-like TAMs. Antibiofouling polymer coatings block
non-specific phagocytosis of IONPs by different cells but enable ligand-mediated
CD206+ M2-like macrophage targeting after surface functionalizing
with mannose (Man-IONP). Costaining tissue sections of the 4T1 mouse
mammary tumors using an anti-CD206 antibody and fluorescent dye (TRITC)-labeled
Man-IONP showed 94.7 ± 4.5% colocalization of TRITC-Man-IONPs
with the anti-CD206 antibody. At 48 h after intravenous (i.v.) injection
of Man-IONPs, magnetic resonance imaging of mice bearing orthotopic
4T1 mammary tumors showed a significantly larger IONP-induced decrease
of the transverse relaxation time (T2)
in tumors with 29.4 ± 1.5 ms compared to 12.3 ± 3.6 ms in
tumors that received non-targeted IONP probes (P <
0.001). Immunofluorescence-stained tumor tissue sections collected
at 6, 18, and 24 h after i.v. administration of the nanoprobes revealed
that Man-IONPs specifically targeted CD206+ M2-like macrophages
in various tumor areas at all time points, while nonconjugated IONPs
were absent in the tumor after 18 h. Thus, antibiofouling Man-IONPs
demonstrated the capability of explicitly imaging CD206+ M2-like macrophages in vivo and potentials for
investigating the dynamics of macrophages in the tumor microenvironment
and delivering therapeutics targeting M2-like TAMs.
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Li, Yuancheng; Wu, Hui; Ji, Bing; Qian, Weiping; Xia, Siyuan; Wang, Liya; et al. (2020). Targeted Imaging of CD206 Expressing Tumor-Associated
M2-like Macrophages Using Mannose-Conjugated Antibiofouling Magnetic
Iron Oxide Nanoparticles. ACS Publications. Collection. https://doi.org/10.1021/acsabm.0c00368
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AUTHORS (10)
YL
Yuancheng Li
HW
Hui Wu
BJ
Bing Ji
WQ
Weiping Qian
SX
Siyuan Xia
LW
Liya Wang
YX
Yaolin Xu
JC
Jing Chen
LY
Lily Yang
HM
Hui Mao
KEYWORDS
M 2-like macrophageCD 206ligand-mediated CD 206Antibiofouling polymer coatings block non-specific phagocytosisAGE polymer-coated iron oxide nanoparticlestumorM 2-like TAMsTRITCPEGM 2-like macrophages4 T 1 mouseanti-CD 206 antibodynon-targeted IONP probesorthotopic 4 T 1Costaining tissue sectionsIron Oxide Nanoparticlesimaging CD 206Man-IONPTumor-Associated M 2-like Macrophages