Surface Modification of Polymers for Tissue Engineering
Applications: Arginine Acts as a Sticky Protein Equivalent for Viable
Cell Accommodation
Posted on 2018-04-16 - 08:29
Hydrophobic polymers,
for their favorable mechanical properties,
are a popular choice as permanent bioimplants. These materials remain
absolutely bioinert for years, but throw up challenges when it comes
to fast integration with healthy tissue. Addressing this, herein,
we present a surface-modification technique of converting the hydrophobic
surface of a polymeric film into a hydrophilic one using a layer-by-layer
assembly process involving gold nanoparticles and small molecules
like amino acids. These films showed much improved animal cell (murine
fibroblast) adherence properties compared to commercially available
tissue culture plates. Moreover, arginine-modified films exhibited
a nearly equivalent cell viability compared to the films modified
with the natural extracellular matrix component fibronectin. The surface
hydrophilicity and roughness of our novel film were characterized
by contact angle measurement and atomic force microscopy. Cell counting,
fluorescence microscopy, cell viability, and collagen estimation assay
were employed to demonstrate that our film favored a much improved
cell adherence, and accommodation in comparison to the commercially
available tissue culture plates.
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Sengupta, Poulomi; L. V. Prasad, Bhagavatula (2018). Surface Modification of Polymers for Tissue Engineering
Applications: Arginine Acts as a Sticky Protein Equivalent for Viable
Cell Accommodation. ACS Publications. Collection. https://doi.org/10.1021/acsomega.8b00215
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AUTHORS (2)
PS
Poulomi Sengupta
BL
Bhagavatula L. V. Prasad
KEYWORDS
Surface Modificationcontact angle measurementequivalent cell viabilityarginine-modified filmssurface hydrophilicityArginine ActsSticky Protein Equivalentanimal celladherence propertiescollagen estimation assaytissue culture platesViable Cell Accommodation Hydrophobic polymersmurine fibroblastlayer-by-layer assembly processextracellular matrix component fibronectinfluorescence microscopycell viabilitysurface-modification techniquenovel filmgold nanoparticlescell adherenceforce microscopyTissue Engineering Applications