Supplementary material from "Zebrafish nephrosin helps host defense against Escherichia coli infection"
Posted on 2017-08-14 - 08:59
Neutrophils play important roles in innate immunity and are mainly dependent on various enzyme-containing granules to kill engulfed microorganisms. Zebrafish nephrosin (npsn) is specifically expressed in neutrophils; however, its function is largely unknown. Here, we generated an npsn mutant (npsnsmu5) via CRISPR/Cas9 to investigate the in vivo function of Npsn. The overall development and number of neutrophils remained unchanged in npsn-deficient mutants, whereas neutrophil antibacterial function was defective. Upon infection with Escherichia coli, the npsnsmu5 mutants exhibited a lower survival rate and more severe bacterial burden, as well as augmented inflammatory response to challenge with infection when compared with wild-type embryos, whereas npsn-overexpressing zebrafish exhibited enhanced host defense against E. coli infection. These findings demonstrated that zebrafish Npsn promotes host defense against bacterial infection. Furthermore, our findings suggested that npsn-deficient and -overexpressing zebrafish might serve as effective models of in vivo innate immunity.
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Di, Qianqian; Lin, Qing; Huang, Zhibin; Chi, Yali; Chen, Xiaohui; ZHANG, Wenqing; et al. (2017). Supplementary material from "Zebrafish nephrosin helps host defense against Escherichia coli infection". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.3852100.v1