Supplementary material from "The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages"
Posted on 2016-11-10 - 13:42
Mycobacterium abscessus is a pathogenic, rapidly growing mycobacterium responsible for pulmonary and cutaneous infections in immunocompetent patients and in patients with Mendelian disorders, such as cystic fibrosis (CF). Mycobacterium abscessus is known to transition from a smooth (S) morphotype with cell surface-associated glycopeptidolipids (GPL) to a rough (R) morphotype lacking GPL. Herein, we show that M. abscessus S and R variants are able to grow inside macrophages and are present in morphologically distinct phagosomes. The S forms are found mostly as single bacteria within phagosomes characterized by a tightly apposed phagosomal membrane and the presence of an electron translucent zone (ETZ) surrounding the bacilli. By contrast, infection with the R form leads to phagosomes often containing more than two bacilli, surrounded by a loose phagosomal membrane and lacking the ETZ. In contrast with the R variant, the S variant is capable of restricting intraphagosomal acidification and induces less apoptosis and autophagy. Importantly, the membrane of phagosomes enclosing the S forms showed signs of alteration such as breaks or partial degradation. Although not frequently encountered, these events suggest that the S form is capable of provoking phagosome-cytosol communications. In conclusion, M. abscessus S exhibits traits inside macrophages that are reminiscent of slow-growing mycobacterial species.
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Roux, Anne-Laure; Viljoen, Albertus; Bah, Aïcha; Simeone, Roxane; Bernut, Audrey; Laencina, Laura; et al. (2016). Supplementary material from "The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.3573255.v1
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AUTHORS (16)
AR
Anne-Laure Roux
AV
Albertus Viljoen
AB
Aïcha Bah
RS
Roxane Simeone
AB
Audrey Bernut
LL
Laura Laencina
TD
Therese Deramaudt
MR
Martin Rottman
JG
Jean-Louis Gaillard
LM
Laleh Majlessi
RB
Roland Brosch
FG
Fabienne Girard-Misguich
IV
Isabelle Vergne
Cd
Chantal de Chastellier
LK
Laurent Kremer
JH
Jean-Louis Herrmann