Supplementary material from "Temperature affects acute mayfly responses to elevated salinity: implications for toxicity of road de-icing salts"

Salinity in freshwater ecosystems has increased significantly at numerous locations throughout the world, and this increase often reflects the use or production of salts from road de-icing, mining/oil and gas drilling activities, or agricultural production. When related to de-icing salts, highest salinity often occurs in winter when water temperature is often low relative to mean annual temperature at a site. Our study examined acute (96 h) responses to elevated salinity (NaCl) concentrations at 5–7 temperature treatments (5–25°C) for four mayfly species (Baetidae: Neocloeon triangulifer, Procloeon fragile; Heptageniidae: Maccaffertium modestum; Leptophlebiidae: Leptophlebia cupida) that are widely distributed across eastern North America. Based on acute LC50s at 20°C, P. fragile was most sensitive (LC50 = 767 mg l⁻¹, 1447 μS cm⁻¹), followed by N. triangulifer (2755 mg l⁻¹, 5104 μS cm⁻¹), M. modestum (2760 mg l⁻¹, 5118 μS cm⁻¹) and L. cupida (4588 mg l⁻¹, 8485 μS cm⁻¹). Acute LC50s decreased as temperature increased for all four species (n = 5–7, R² = 0.65–0.88, p = 0.052–0.002). Thus, acute salt toxicity is strongly temperature dependent for the mayfly species we tested, which suggests that brief periods of elevated salinity during cold seasons or in colder locations may be ecologically less toxic than predicted by standard 20 or 25°C laboratory bioassays.This article is part of the theme issue ‘Salt in freshwaters: causes, ecological consequences and future prospects’.