Supplementary material from "Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial symbionts mediates their joint transovarial transmission"

Published on 2018-12-06T16:00:31Z (GMT) by
Many hemipteran insects that can transmit plant viruses in a persistent and transovarial manner are generally associated with a common obligate bacterial symbiont <i>Sulcia</i> and its β-proteobacterial partner. Rice dwarf virus (RDV), a plant reovirus, can bind to the envelope of <i>Sulcia</i> through direct interaction of the viral minor outer capsid protein P2 with the bacterial outer membrane protein, allowing the virus to exploit the ancient oocyte entry path of <i>Sulcia</i> in rice leafhopper vectors. Here, we show that RDV can hitchhike with both <i>Sulcia</i> and its β-proteobacterial partner <i>Nasuia</i> to ensure their simultaneous transovarial transmission. Interestingly, RDV can move through the outer envelope of <i>Nasuia</i> and reside in the periplasmic space, which is mediated by the specific interaction of the viral major outer capsid protein P8 and the porin channel on the bacterial outer envelope. <i>Nasuia</i> porin-specific antibody efficiently interferes with the binding between RDV and the <i>Nasuia</i> envelope, thus strongly preventing viral transmission to insect offspring. Thus, RDV has evolved different strategies to exploit the ancient oocyte entry paths used by two obligate bacterial symbionts in rice leafhoppers. Our results thus reveal that RDV has formed complex, cooperative interactions with both <i>Sulcia</i> and <i>Nasuia</i> during their joint transovarial transmission.This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.

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Wu, Wei; Huang, Lingzhi; Mao, Qianzhuo; Wei, Jing; Li, Jiajia; Zhao, Yu; et al. (2018): Supplementary material from "Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial symbionts mediates their joint transovarial transmission". The Royal Society. Collection.