Supplementary material from "Expression and function of ATP-dependent potassium channels in zebrafish islet β-cells"

ATP-sensitive potassium channels (K_ATP channels) are critical nutrient sensors in many mammalian tissues. In the pancreas, K_ATP channels are essential for coupling glucose metabolism to insulin secretion. While orthologous genes for many components of metabolism–secretion coupling in mammals are present in lower vertebrates, their expression, functionality and ultimate impact on body glucose homeostasis are unclear. In this paper, we demonstrate that zebrafish islet β-cells express functional K_ATP channels of similar subunit composition, structure and metabolic sensitivity to their mammalian counterparts. We further show that pharmacological activation of native zebrafish K_ATP using diazoxide, a specific K_ATP channel opener, is sufficient to disturb glucose tolerance in adult zebrafish. That β-cell K_ATP channel expression and function are conserved between zebrafish and mammals illustrates the evolutionary conservation of islet metabolic sensing from fish to humans, and lends relevance to the use of zebrafish to model islet glucose sensing and diseases of membrane excitability such as neonatal diabetes.