Supplementary material from "Effect of genetic drift on determinants of protein evolution"

Published on 2018-07-11T09:31:11Z (GMT) by
A number of previous studies reported that gene expression, tissue specificity, gene essentiality and the number of protein–protein interactions influence the rate of protein evolution. Here we investigated the influence of effective population size (<i>N</i><sub>e</sub>) on these determinants of protein evolution. For this purpose, we compared the ratio of non-synonymous-to-synonymous diversities (<i>π</i><sub>N</sub>/<i>π</i><sub>S</sub>) estimated for protein-coding genes of <i>Mus musculus castaneus</i> and <i>Mus musculus musculus</i>: populations with high and low <i>N</i><sub>e</sub> respectively. Our results revealed that the difference between <i>π</i><sub>N</sub>/<i>π</i><sub>S</sub> estimated for genes with high and low expression levels was significantly smaller for <i>M. m. musculus</i> compared to that observed for <i>M. m. castaneus</i>. The difference between the <i>π</i><sub>N</sub>/<i>π</i><sub>S</sub> of broadly expressed and tissue specific genes was much higher for <i>M. m. castaneus</i> compared to that of <i>M. m. musculus.</i> Furthermore, the difference between the <i>π</i><sub>N</sub>/<i>π</i><sub>S</sub> computed for essential and non-essential genes was much smaller for <i>M. m. musculus</i> than <i>M. m. castaneus</i>. A similar pattern was observed for genes involved in many protein–protein interactions versus those involved in one. These results suggest that the effects of the determinants on protein evolution were much reduced for the population with small <i>N</i><sub>e</sub> due to increased genetic drift.

Cite this collection

Subramanian, Sankar (2018): Supplementary material from "Effect of genetic drift on determinants of protein evolution". The Royal Society. Collection.