Supplementary material from "Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection"
Posted on 2017-10-16 - 09:17
Successful generation of virions from infected cells is a complex process requiring orchestrated regulation of host and viral genes. Cells infected with human cytomegalovirus undergo a dramatic reorganization of membrane organelles resulting in the formation of the virion assembly compartment, a process that is not fully understood. Here we show that acidification of vacuoles by the cellular v-ATPase is a crucial step in the formation of the virion assembly compartment and disruption of acidification results in mis-localization of virion components and a profound reduction in infectious virus levels. In addition, knockdown of ATP6V0C blocks the increase in nuclear size, normally associated with HCMV infection. Inhibition of the v-ATPase does not affect intracellular levels of viral DNA synthesis or gene expression, consistent with a defect in assembly and egress. These studies identify a novel host factor involved in virion production and a potential target for antiviral therapy.
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Pavelin, Jonathan; McCormick, Dominique; Chiweshe, Stephen; Ramachandran, Saranya; Lin, Yao-Tang; Grey, Finn (2017). Supplementary material from "Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.3904894.v1