Isoforms of Spectrin and Ankyrin Reflect the Functional Topography of the Mouse Kidney
Posted on 2016-01-04 - 11:20
Spectrin and ankyrin mRNA expression were measured by RT-PCR and qPCR with primers summarized in Table 1. (A) Amplimers were detected for spectrins βΙ, βΙΙ, βΙΙΙ, and αΙΙ and for ankyrins R (Ank1), G (Ank2), and B (Ank3). NC is no-RNA control. (B) The levels of the various transcripts varied widely, as measured by qPCR. Relative abundance is presented. Results shown for three separate determinations. Error bars ±1SD from mean. (Inset) RT-PCR detected two alternative transcripts of βΙΙ spectrin (βΙΙΣ1 & βΙΙΣ2), but only one of two potential transcripts of βΙ spectrin.
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C. Stankewich, Michael; W. Moeckel, Gilbert; Ji, Lan; Ardito, Thomas; Morrow, Jon S. (2016). Isoforms of Spectrin and Ankyrin Reflect the Functional Topography of the Mouse Kidney. PLOS ONE. Collection. https://doi.org/10.1371/journal.pone.0142687