Spatiotemporal Dynamics of Synthetic Microbial Consortia
in Microfluidic Devices
Version 3 2019-08-15, 16:36Version 3 2019-08-15, 16:36
Version 2 2019-08-09, 18:39Version 2 2019-08-09, 18:39
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Posted on 2019-08-15 - 16:36
Synthetic microbial
consortia consist of two or more engineered
strains that grow together and share the same resources. When intercellular
signaling pathways are included in the engineered strains, close proximity
of the microbes can generate complex dynamic behaviors that are difficult
to obtain using a single strain. However, when a consortium is not
cultured in a well-mixed environment the constituent strains passively
compete for space as they grow and divide, complicating cell–cell
signaling. Here, we explore the temporal dynamics of the spatial distribution
of consortia cocultured in microfluidic devices. To do this, we grew
two different strains of Escherichia coli in microfluidic
devices with cell-trapping regions (traps) of several different designs.
We found that the size of the traps is a critical determinant of spatiotemporal
dynamics. In small traps, cells can easily signal one another, but
the relative proportion of each strain within the trap can fluctuate
wildly. In large traps, the relative ratio of strains is stabilized,
but intercellular signaling can be hindered by distances between cells.
This presents a trade-off between the trap size and the effectiveness
of intercellular signaling, which can be mitigated by increasing the
initial seeding of cells in larger traps. We also built a mathematical
model, which suggests that increasing the number of seed cells can
also increase the strain ratio variability due to an increased number
of strain interfaces in the trap. These results help elucidate the
complex behaviors of synthetic microbial consortia in microfluidic
traps and provide a means of analysis to help remedy the spatial heterogeneity
inherent to different trap types.
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Alnahhas, Razan
N.; Winkle, James J.; Hirning, Andrew J.; Karamched, Bhargav; Ott, William; Josić, Krešimir; et al. (2019). Spatiotemporal Dynamics of Synthetic Microbial Consortia
in Microfluidic Devices. ACS Publications. Collection. https://doi.org/10.1021/acssynbio.9b00146