Spatially Constrained DNA Nanomachines To Accelerate
Kinetics in Response to External Input: Design and Bioanalysis
Posted on 2020-06-25 - 18:13
Cells take advantage
of the spatial organization to accelerate
the reaction kinetics of diverse components within a crowded intracellular
environment. Inspired by this, we hereby designed a principle of spatial
constraint to overcome limitations of response kinetics in DNAzyme-powered
DNA nanomachines. First, we proposed the type-1 of spatially constrained
DNA nanomachines (scDN-1) by co-localizing the aptamer probe and power
unit (DNAzyme), allowing the DNA nanomachines to accomplish faster
cyclic cleavage of DNAzyme as intramolecular reactions. To expand
the scDN into the clinical practice, Type 2 spatially constrained
DNA nanomachines (scDN-2) with constrained antibody probes were then
constructed through Holliday junction assembly, which increased the
effective local concentration to obtain the improved kinetics. With
an accelerated response kinetics, this design principle allows DNA
nanomachines to accomplish the response to tumor markers in real patients’
samples within 30 min, significantly broadening the bioanalytical
applications of DNA nanomachines to clinical practice.
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Zhou, Rongxing; Hu, Changjia; Jin, Yanwen; Zhang, Jie; Du, Huan; Yang, Peng; et al. (2020). Spatially Constrained DNA Nanomachines To Accelerate
Kinetics in Response to External Input: Design and Bioanalysis. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.0c00802