Single-Neuron Comparison of the Olfactory Receptor
Response to Deuterated and Nondeuterated Odorants
Posted on 2018-10-20 - 00:00
The mammalian olfactory receptors
(ORs) constitute a large subfamily
of the Class A G-protein coupled receptors (GPCRs). The molecular
details of how these receptors convert odorant chemical information
into neural signal are unknown, but are predicted by analogy to other
GPCRs to involve stabilization of the activated form of the OR by
the odorant. An alternative hypothesis maintains that the vibrational
modes of an odorant’s bonds constitute the main determinant
for OR activation, and that odorants containing deuterium in place
of hydrogen should activate different sets of OR family members. Experiments
using heterologously expressed ORs have failed to show different responses
for deuterated odorants, but experiments in the sensory neuron environment
have been lacking. We tested the response to deuterated and nondeuterated
versions of p-cymene, 1-octanol, 1-undecanol, and
octanal in dissociated mouse olfactory receptor neurons (ORNs) by
calcium imaging. In all, we tested 23 812 cells, including
a subset expressing recombinant mouse olfactory receptor 2 (Olfr2/OR-I7), and found that nearly all
of the 1610 odorant-responding neurons were unable to distinguish
the D- and H-odorants. These results support the conclusion that if
mammals can perceive deuterated odorants differently, the difference
arises from the receptor-independent steps of olfaction. Nevertheless,
0.81% of the responding ORNs responded differently to D- and H-odorants,
and those in the octanal experiments responded selectively to H-octanal
at concentrations from 3 to 100 μM. The few ORs responding differently
to H and D may be hypersensitive to one of the several H/D physicochemical
differences, such as the difference in H/D hydrophobicity.
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Na, Mihwa; Liu, Min Ting; Q. Nguyen, Minh; Ryan, Kevin (2018). Single-Neuron Comparison of the Olfactory Receptor
Response to Deuterated and Nondeuterated Odorants. ACS Publications. Collection. https://doi.org/10.1021/acschemneuro.8b00416
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AUTHORS (4)
MN
Mihwa Na
ML
Min Ting Liu
MQ
Minh Q. Nguyen
KR
Kevin Ryan
KEYWORDS
odorant chemical informationGPCRcalcium imagingreceptor 2receptor neurons23 812ORSingle-Neuron Comparisonvibrational modesoctanal experimentsOlfactory Receptor Responseneuron environmentresults support100 μ Mnondeuterated versions23 812 cellsalternative hypothesis1- undecanolORNOR-I1610 odorant-responding neurons1- octanolreceptor-independent stepsNondeuterated Odorantsfamily members