Single-Atom
Fluorescence Switch: A General Approach
toward Visible-Light-Activated
Dyes for Biological Imaging
Posted on 2019-09-06 - 21:29
Photoactivatable
fluorophores afford powerful molecular tools to
improve the spatial and temporal resolution of subcellular structures
and dynamics. By performing a single sulfur-for-oxygen atom replacement
within common fluorophores, we have developed a facile and general
strategy to obtain photoactivatable fluorogenic dyes across a broad
spectral range. Thiocarbonyl substitution within fluorophores results
in significant loss of fluorescence via a photoinduced electron transfer-quenching
mechanism as suggested by theoretical calculations. Significantly,
upon exposure to air and visible light residing in their absorption
regime (365–630 nm), thio-caged fluorophores can be efficiently
desulfurized to their oxo derivatives, thus restoring strong emission
of the fluorophores. The effective photoactivation makes thio-caged
fluorophores promising candidates for super-resolution imaging, which
was realized by photoactivated localization microscopy (PALM) with
low-power activation light under physiological conditions in the absence
of cytotoxic additives (e.g., thiols, oxygen scavengers), a feature
superior to traditional PALM probes. The versatility of this thio-caging
strategy was further demonstrated by multicolor super-resolution imaging
of lipid droplets and proteins of interest.
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Tang, Juan; Robichaux, Michael A.; Wu, Kuan-Lin; Pei, Jingqi; Nguyen, Nhung T.; Zhou, Yubin; et al. (2019). Single-Atom
Fluorescence Switch: A General Approach
toward Visible-Light-Activated
Dyes for Biological Imaging. ACS Publications. Collection. https://doi.org/10.1021/jacs.9b06237
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AUTHORS (8)
JT
Juan Tang
MR
Michael A. Robichaux
KW
Kuan-Lin Wu
JP
Jingqi Pei
NN
Nhung T. Nguyen
YZ
Yubin Zhou
TW
Theodore G. Wensel
HX
Han Xiao