Roles of Active-Site Aromatic Residues in Cold Adaptation
of Sphingomonas glacialis Esterase
EstSP1
Version 2 2017-12-08, 09:14
Version 1 2017-12-07, 20:18
Posted on 2017-12-08 - 09:14
The aromatic amino acids, Tyr or
Trp, which line the active-site
walls of esterases, stabilize the catalytic His loop via hydrogen
bonding. A Tyr residue is preferred in extremophilic esterases (psychrophilic
or hyperthermophilic esterases), whereas a Trp residue is preferred
in moderate-temperature esterases. Here, we provide evidence that
Tyr and Trp play distinct roles in cold adaptation of the psychrophilic
esterase EstSP1 isolated from an Arctic bacterium Sphingomonas
glacialis PAMC 26605. Stern–Volmer plots showed
that the mutation of Tyr191 to Ala, Phe, Trp, and His resulted in
reduced conformational flexibility of the overall protein structure.
Interestingly, the Y191W and Y191H mutants showed increased thermal
stability at moderate temperatures. All Tyr191 mutants showed reduced
catalytic activity relative to wild-type EstSP1. Our results indicate
that Tyr with its phenyl hydroxyl group is favored for increased conformational
flexibility and high catalytic activity of EstSP1 at low temperatures
at the expense of thermal stability. The results of this study suggest
that, in the permanently cold Arctic zone, enzyme activity has been
selected for psychrophilic enzymes over thermal stability. The results
presented herein provide novel insight into the roles of Tyr and Trp
residues for temperature adaptation of enzymes that function at low,
moderate, and high temperatures.
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Kashif, Ayesha; Tran, Lan-Huong; Jang, Sei-Heon; Lee, ChangWoo (2017). Roles of Active-Site Aromatic Residues in Cold Adaptation
of Sphingomonas glacialis Esterase
EstSP1. ACS Publications. Collection. https://doi.org/10.1021/acsomega.7b01435
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AUTHORS (4)
AK
Ayesha Kashif
LT
Lan-Huong Tran
SJ
Sei-Heon Jang
CL
ChangWoo Lee
KEYWORDS
Tyr 191 mutantsstabilityenzyme activityY 191WArctic zoneactive-site wallsCold Adaptationwild-type EstSP 1.psychrophilic enzymesmoderate-temperature esterasesextremophilic esterasestemperature adaptationTyr 191phenyl hydroxyl groupTrp residueTyr residueprotein structureSphingomonas glacialis Esterase EstSP 1Trp residuesY 191H mutantsnovel insightPAMCActive-Site Aromatic ResiduesEstSP 1psychrophilic esterase EstSP 1