Reflection phase microscopy using spatio-temporal coherence of light

Published on 2018-11-13T20:11:47Z (GMT) by
Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and 3D resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. Reflection phase microscope can potentially elucidate biomechanic mechanisms of pathological and physiological processes.

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Choi, Youngwoon; Hosseini, Poorya; Kang, Jeon Woong; Kang, Sungsam; Yang, Taeseok; Hyeon, Min Gyu; et al. (2018): Reflection phase microscopy using spatio-temporal coherence of light. The Optical Society. Collection.