Quantification of Molecular Interactions in Living
Cells in Real Time using a Membrane Protein Nanopattern
Posted on 2020-06-11 - 11:40
Molecular
processes within cells have traditionally been studied
with biochemical methods due to their high degree of specificity and
ease of use. In recent years, cell-based assays have gained more and
more popularity since they facilitate the extraction of mode of action,
phenotypic, and toxicity information. However, to provide specificity,
cellular assays rely heavily on biomolecular labels and tags while
label-free cell-based assays only offer holistic information about
a bulk property of the investigated cells. Here, we introduce a cell-based
assay for protein–protein interaction analysis. We achieve
specificity by spatially ordering a membrane protein of interest into
a coherent pattern of fully functional membrane proteins on the surface
of an optical sensor. Thereby, molecular interactions with the coherently
ordered membrane proteins become visible in real time, while nonspecific
interactions and holistic changes within the living cell remain invisible.
Due to its unbiased nature, this new cell-based detection method presents
itself as an invaluable tool for cell signaling research and drug
discovery.
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Reichmuth, Andreas
Michael; Zimmermann, Mirjam; Wilhelm, Florian; Frutiger, Andreas; Blickenstorfer, Yves; Fattinger, Christof; et al. (2020). Quantification of Molecular Interactions in Living
Cells in Real Time using a Membrane Protein Nanopattern. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.0c00987
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AUTHORS (8)
AR
Andreas
Michael Reichmuth
MZ
Mirjam Zimmermann
FW
Florian Wilhelm
AF
Andreas Frutiger
YB
Yves Blickenstorfer
CF
Christof Fattinger
MW
Maria Waldhoer
JV
János Vörös