PySight: plug and play photon counting for fast continuous volumetric intravital microscopy
Published on 2018-09-13T13:31:33Z (GMT) by
Imaging increasingly large neuronal populations at high rates pushed multi-photon microscopy into the photon-deprived regime. We present PySight, an add-on hardware and software solution tailored for photon-deprived imaging conditions. PySight more than triples the median amplitude of neuronal calcium transients in awake mice, and facilitates single-trial intravital voltage imaging in fruit flies. Its unique data streaming architecture allowed us to image a fruit fly's olfactory response over 234 · 600 · 330 μm^3 at 73 volumes per second, outperforming top-tier imaging setups while retaining over 200 times lower data rates. PySight requires no electronics expertise nor custom synchronization boards, and its open-source software is extensible to any imaging method based on single-pixel (bucket) detectors. PySight offers an optimal data acquisition scheme for ever increasing imaging volumes of turbid living tissue.
Cite this collection
Har-Gil, Hagai; Golgher, Lior; Israel, Shai; Kain, David; Cheshnovsky, Ori; Parnas, Moshe; et al. (2018): PySight: plug and play photon counting for fast continuous volumetric intravital microscopy. The Optical Society. Collection.