Proteomic Analysis
of the Promastigote Secretome of
Seven Leishmania Species
Posted on 2021-11-22 - 16:09
Leishmaniasis is one of the most
impactful parasitic diseases worldwide,
endangering the lives of 1 billion people every year. There are 20
different species of Leishmania able to infect humans,
causing cutaneous (CL), visceral (VL), and/or mucocutaneous leishmaniasis
(MCL). Leishmania parasites are known to secrete
a plethora of proteins to establish infection and modulate the host’s
immune system. In this study, we analyzed using tandem mass spectrometry
the total protein content of the secretomes produced by promastigote
forms from seven Leishmania species grown in serum-free in vitro cultures. The core secretome shared by all seven Leishmania species corresponds to up to one-third of total
secreted proteins, suggesting conserved mechanisms of adaptation to
the vertebrate host. The relative abundance confirms the importance
of known virulence factors and some proteins uniquely present in CL-
or VL-causing species and may provide further insight regarding their
pathogenesis. Bioinformatic analysis showed that most proteins were
secreted via unconventional mechanisms, with an important role for
vesicle-based secretion for all species. Gene Ontology annotation
and enrichment analyses showed a high level of functional conservation
among species. This study contributes to the current knowledge on
the biological significance of differently secreted proteins and provides
new information on the correlation of Leishmania secretome
to clinical outcomes and species-specific pathogenesis.
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Pissarra, Joana; Pagniez, Julie; Petitdidier, Elodie; Séveno, Martial; Vigy, Oana; Bras-Gonçalves, Rachel; et al. (2021). Proteomic Analysis
of the Promastigote Secretome of
Seven Leishmania Species. ACS Publications. Collection. https://doi.org/10.1021/acs.jproteome.1c00244
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AUTHORS (8)
JP
Joana Pissarra
JP
Julie Pagniez
EP
Elodie Petitdidier
MS
Martial Séveno
OV
Oana Vigy
RB
Rachel Bras-Gonçalves
JL
Jean-Loup Lemesre
PH
Philippe Holzmuller
KEYWORDS
total protein contentsuggesting conserved mechanismsrelative abundance confirmsprovides new informationgene ontology annotationenrichment analyses showedtotal secreted proteinsproteins uniquely presentdifferently secreted proteinsbioinformatic analysis showedknown virulence factorscore secretome shared20 different speciescl ), visceralproteomic analysisvl ),vitro vertebrate hostspecies leishmaniasisspecies grownspecies correspondssecretomes producedpromastigote secretomepromastigote formsmucocutaneous leishmaniasismcl ).may provideleishmania insight regardinginfect humansimportant roleimmune systemhost ’high levelestablish infectioncurrent knowledgeclinical outcomescausing speciescausing cutaneousbiological significancebased secretion