Plasmonic Nanosensor Array for Multiplexed DNA-based
Pathogen Detection
Posted on 2019-01-18 - 00:00
In
this research we introduce a plasmonic nanoparticle based optical
biosensor for monitoring of molecular binding events. The sensor utilizes
spotted gold nanoparticle arrays as sensing platform. The nanoparticle
spots are functionalized with capture DNA sequences complementary
to the analyte (target) DNA. Upon incubation with the target sequence,
it will bind on the respectively complementary functionalized particle
spot. This binding changes the local refractive index, which is detected
spectroscopically as the resulting changes of the localized surface
plasmon resonance (LSPR) peak wavelength. In order to increase the
signal, a small gold nanoparticle label is introduced. The binding
can be reversed using chemical means (10 mM HCl). It is demonstrated
that multiplexed detection and identification of several fungal pathogen
DNA sequences subsequently on one sensor array are possible by this
approach.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Zopf, David; Pittner, Angelina; Dathe, André; Grosse, Norman; Csáki, Andrea; Arstila, Kai; et al. (2019). Plasmonic Nanosensor Array for Multiplexed DNA-based
Pathogen Detection. ACS Publications. Collection. https://doi.org/10.1021/acssensors.8b01073
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (12)
DZ
David Zopf
AP
Angelina Pittner
AD
André Dathe
NG
Norman Grosse
AC
Andrea Csáki
KA
Kai Arstila
JT
J. Jussi Toppari
WS
Walter Schott
DD
Denis Dontsov
GU
Günter Uhlrich
WF
Wolfgang Fritzsche
OS
Ondrej Stranik
KEYWORDS
plasmonic nanoparticlesurface plasmon resonancegold nanoparticle arraysLSPRpeak wavelengthgold nanoparticle labelDNA sequencesPlasmonic Nanosensor Arraysensor arraynanoparticle spotsfunctionalized particle spotpathogen DNA sequencesbinding eventsmultiplexed detectiontarget sequencebinding changesMultiplexed DNA-based Pathogen Detection