Novel ECM Patch Combines Poly(vinyl
alcohol), Human Fibroblast-Derived Matrix, and Mesenchymal Stem Cells
for Advanced Wound Healing
Posted on 2020-06-22 - 11:35
Decellularized extracellular
matrix (ECM)-based scaffold has been a very useful resource for effective
tissue regeneration. In this study, we report a novel ECM patch that
physically combines human fibroblast-derived matrix (hFDM) and poly(vinyl
alcohol) (PVA) hydrogel. hFDM was obtained after decellularization
of in vitro cultured human fibroblasts. We investigated
the basic characteristics of hFDM
alone using immunofluorescence (fibronectin, collagen type I) and
angiogenesis-related factor analysis. Successful incorporation of
hFDM with PVA produced an hFDM/PVA patch, which showed excellent cytocompatibility
with human mesenchymal stem cells (hMSCs), as assessed via cell adhesion,
viability, and proliferation. Moreover, in vitro scratch
assay using human dermal fibroblasts showed a significant improvement
of cell migration when treated with the paracrine factors originated
from the hMSC-incorporated hFDM. To evaluate the therapeutic effect
on wound healing, hMSCs were seeded on the hFDM/PVA patch and they
were then transplanted into a mouse full-thickness wound model. Among
four experimental groups (control, PVA, hFDM/PVA, hMSC/hFDM/PVA),
we found that hMSC/hFDM/PVA patch accelerated the wound closure with
time. More notably, histology and immunofluorescence demonstrated
that compared to the other interventions tested, hMSC/hFDM/PVA patch
could lead to significantly advanced tissue regeneration, as confirmed
via nearly normal epidermis thickness, skin adnexa regeneration (hair
follicle), mature collagen deposition, and neovascularization. Additionally,
cell tracking of prelabeled hMSCs suggests the in vivo retention of transplanted cells in the wound region after the transplantation
of hMSC/hFDM/PVA patch. Taken together, our engineered ECM patch supports
a strong regenerative potential toward advanced wound healing.
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Ha, Sang Su; Song, Eui Sun; Du, Ping; Suhaeri, Muhammad; Lee, Jong Ho; Park, Kwideok (2020). Novel ECM Patch Combines Poly(vinyl
alcohol), Human Fibroblast-Derived Matrix, and Mesenchymal Stem Cells
for Advanced Wound Healing. ACS Publications. Collection. https://doi.org/10.1021/acsbiomaterials.0c00657
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AUTHORS (6)
SH
Sang Su Ha
ES
Eui Sun Song
PD
Ping Du
MS
Muhammad Suhaeri
JL
Jong Ho Lee
KP
Kwideok Park